Question 1 Not yet graded / 2.5 pts Question 2 Not yet graded / 2.5 pts Question 3 Not yet graded / 2.5 pts BIOD 171 Microbiology Lab Q & A
(Latest 2023 / 2024)
- 1 - 9 ) Exam Portage Learning Bundle
BIOD 171
L1: Lab Exam 1 2023 / 2024
If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you choose to best sterilize your equipment? Hot steam or hot air?Explain why you chose your answer.
Your Answer:
Hot steam. Hot steam is more efficient because it takes a couple of minutes to sterilize and dry air takes a couple of hours to sterilize.Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes whereas hot air will take several hours to achieve the same effect.
What three elements are used in an autoclave to sterilize equipment?
Your Answer:
heat, pressure, steam heat, pressure, and steam
What type of incubator is pictured below? 1 / 4
Question 4 Not yet graded / 2.5 pts Question 5 Not yet graded / 2 pts Question 6 Not yet graded / 2 pts
Your Answer:
Fixed incubator Fixed incubator
What is the minimum temperature an autoclave must be set at to achieve sterile condition?
Your Answer:
125 o C
125°C
At what temperature is the fixed incubator set to, as presented in the lab video?
Your Answer:
37 o C 37°C
What are the main sections that should be found in a lab notebook? Name at least 4. 2 / 4
Question 7 Not yet graded / 2 pts Question 8 Not yet graded / 2 pts Question 9 Not yet graded / 2 pts
Your Answer:
Title Objectives Procedures Notes Results Objective, Procedure, Notes, Results and Deviations
What are the FOUR types of gloves presented in the lab video?
Your Answer:
latex nitrile thermal cold thermal heat Latex, Nitrile, Thermal cold, Thermal heat
At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
Your Answer:
4 o C slows the bacterial growth which helps preserve samples and keep them for long term.4°C. This temperature slows bacterial growth and prolongs the life of the sample.
What THREE rules were discussed in regards to lab safety that would protect you and others from contamination?
Your Answer: 3 / 4
Question 10 Not yet graded / 5 pts Question 11 Not yet graded / 5 pts Never eat or drink in the lab Use personal protective equipment (PPE) Never leave the lab wearing PPE
- Never eat or drink in the lab
- Always wear appropriate PPE
- Never leave the lab wearing PPE
You are a lab instructor, and Sally Miller has turned in her lab notebook for you to grade the lab experiment #1 on lab safety. Based upon what was covered in the lab video, how should Sally have titled her experiment?
Your Answer:
SM01 Lab Safety SM01 Lab Safety
You arrive to your first day of work at a new lab. You are taking over for someone who took a new job at another lab. Your boss informs you that because of time restraints, this person did not exactly follow the experimental protocol. In order to proceed, you must know what he did differently. (1) According to the lab lecture, under what section of his notebook would you look to find the experimental steps? (2) As changes to the experimental steps were made, what are these differences called, and how should it appear in the lab notebook?
Your Answer:
- Procedures
- Deviations. They should be recorded in red to bring attention to the changes
- / 4
(1) Procedure—this is where the steps for the experimental protocol are recorded.(2) Deviations. All deviations should be written in red to immediately bring attention to the changes in the protocol.