1. TFThe term ubiquitous can be applied to every bacterial species

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BIOS 280 LAB

• T/FThe term "ubiquitous" can be applied to every bacterial species

–Answer False

• T/F It is important to wash your hands with soap and water upon entering the laboratory, but

unnecessary before leaving the laboratory.–Answer False

• The lens which is located directly below the microscope stage is called the

–Answer condenser

• T/F When streaking a plate for isolation of colonies ("streak plate tech- nique"), you should

incinerate the loop between each section streaked.–Answer True

• A microorganism that is capable of causing disease in a humans is termed-

–Answer pathogenic

• If you have the 40X objective lens in place and each of the ocular lenses has a magnification

power of 5X, what is the total magnification of the image?–Answer

- 200X

• T/F Microscope lenses can routinely be cleaned with bibulous paper

–Answer False

• You have a 35X objective lens in place, and the numerical aperture of the objective lens is

0.75. The numerical aperture of the condenser lens is 1.25. What is the total magnification if the 1 / 2

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ocular lens is 10X, with the eyepieces spaced 3 cm apart?–Answer 350X

• T/F Bacterial and fungal colonies are easily distinguished by their appear- ance on trypticase

soy agar (TSA) plates –Answer True

• T/F When moving from a low power objective lens to a higher power objective lens, the

field of view that is observed decreases –Answer True

• T/F A turbid broth culture indicates bacterial growth

–Answer True

• T/F Heat fixation is an important part of the preparation of a bacterial smear

–Answer True

• T/F Microorganisms are ubiquitous and can be isolated from soil, water, plants, and

animals –Answer True

• T/F A contaminated culture contains two or more known bacterial species-

–Answer False

• T/F Most bacterial cell surfaces are negatively charged and basic stains that are positively

charged easily color the cells –Answer True

• What are the reagents needed for the Gram stain procedure?
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