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ACS BIOCHEMISTRY EXAM/ Advanced ACS Biochemistry Latest Questions with Correct Verified Answers 2024- 2025/ Rated A+
Henderson-Hasselbach Equation - ANSWER - pH = pKa + log ([A-] / [HA])
FMOC Chemical Synthesis - ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus.
Salting Out (Purification) - ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution.
Size-Exclusion Chromatography - ANSWER - Separates sample based on size with smaller molecules eluting later.
Ion-Exchange Chromatography - ANSWER - Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove stuck proteins.
Hydrophobic/Reverse Phase Chromatography - ANSWER - Beads are coated with a carbon chain.Hydrophobic proteins stick better. Elute with non-H-bonding solvent (acetonitrile).
Affinity Chromatography - ANSWER - Attach a ligand that binds a protein to a bead. Elute with harsh chemicals or similar ligand.
SDS-PAGE - ANSWER - Uses SDS. Gel is made from cross-linked polyacrylamide. Separates based off of mass with smaller molecules moving faster. Visualized with Coomassie blue.
SDS - ANSWER - Sodium dodecyl sulfate. Unfolds proteins and gives them uniform negative charge.
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pg. 2 Isoelectric Focusing - ANSWER - Variation of gel electrophoresis where protein charge matters. Involves electrodes and pH gradient. Protein stops at their pI when neutral.
FDNB (1-fluoro-2,3-dinitrobenzene) - ANSWER - FDNB reacts with the N-terminus of the protein to produce a 2,4-dinitrophenol derivative that labels the first residue. Can repeat hydrolysis to determine sequential amino acids.
DTT (dithiothreitol) - ANSWER - Reduces disulfide bonds.
Iodoacetate - ANSWER - Adds carboxymethyl group on free -SH groups. Blocks disulfide bonding.
Homologs - ANSWER - Shares 25% identity with another gene
Orthologs - ANSWER - Similar genes in different organisms
Paralogs - ANSWER - Similar "paired" genes in the same organism
Ramachandran Plot - ANSWER - Shows favorable phi-psi angle combinations. 3 main "wells" for α- helices, ß-sheets, and left-handed α-helices.
Glycine Ramachandran Plot - ANSWER - Glycine can adopt more angles. (H's for R-group).
Proline Ramachandran Plot - ANSWER - Proline adopts fewer angles. Amino group is incorporated into a ring.
α-helices - ANSWER - Ala is common, Gly & Pro are not very common. Side-chain interactions every 3 or
- residues. Turns once every 3.6 residues. Distance between backbones is 5.4Å.
Helix Dipole - ANSWER - Formed from added dipole moments of all hydrogen bonds in an α-helix. N- terminus is δ+ and C-terminus is δ-.
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pg. 3 ß-sheet - ANSWER - Either parallel or anti-parallel. Often twisted to increase strength.
Anti-parallel ß-sheet - ANSWER - Alternating sheet directions (C & N-termini don't line-up). Has straight H-bonds.
Parallel ß-sheet - ANSWER - Same sheet directions (C & N-termini line up). Has angled H-bonds.
ß-turns - ANSWER - Tight u-turns with specific phi-psi angles. Must have gly at position 3. Proline may also be at ß-turn because it can have a cis-omega angle.
Loops - ANSWER - Not highly structured. Not necessary highly flexible, but can occasionally move. Very variable in sequence.
Circular Dichroism - ANSWER - Uses UV light to measure 2° structure. Can be used to measure destabilization.
Disulfide-bonds - ANSWER - Bonds between two -SH groups that form between 2° and 3° structure.
ß-mercaptoethanol - ANSWER - Breaks disulfide bonds.
α-keratin - ANSWER - formed from 2 α-helices twisted around each other. "Coiled coil". Cross-linked by disulfide bonds.
Collagen - ANSWER - Repeating sequence of Gly-X-Pro. 3 stranded "coiled coil". Contains gly core.
Myoglobin 4° Structure - ANSWER - Symmetric homodimer,
Hemoglobin 4° Structure - ANSWER - Tetramer. Dimer of dimers. α2ß2 tetramer.
α/ß Protein Folding - ANSWER - Less distinct areas of α and ß folding.
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