BIOS 242 Lab Practical

SUBSCRIBE

BIOS 242 Lab Practical

• marcescens

–Answer can grow aerobically and anaerobically

• To prevent contamination from outside sources

–Answer why is proper aseptic tech- nique important?

• heats it up to the point that if there was any bacteria it would be killed

–Answer whats the point of flaming the inoculating loop?

• it might kill the bacteria you are trying to retrieve

–Answer what happens if you put the hot loop into the tube too soon?

• to penetrate the agar to allow for bacteria to grow anaerobically

–Answer why do you stab the agar?

• breakout of a disease

–Answer what happens if there is a breakdown in aseptic tech- nique?

• circular

–Answer shape of s. marcescens

• circular

–Answer shape of e. coli

• umbonate

–Answer elevation of s. marcescens 1 / 2

SUBSCRIBE

• limit the number of bacteria in each quadrant (isolate colonies)

–Answer why do you streak the four quadrants?

• total magnification

–Answer magnification of objective lens x magnification of ocular lens

• 200x

–Answer 20x objective and 10x eyepiece

• 750x

–Answer 50x objective x 15x eyepiece

• carbolfuchsin

–Answer acid fast primary stain

• acid fast stain

–Answer used for organisms that don't stain well with gram due to the waxy outer layer

• increased magnification decreases field of vision

–Answer what does increasing magnification do to field of vision?

• more light is going through your slide thus making it a clearer image

–Answer why is oil immersion necessary at 100x?

• 100x

–Answer when do you use oil immersion?

• parfocal

–Answer means that you can change the focus (objective lens) of a microscope without having to focus it

• / 2