BIOS 280 FINAL Exam - 2. gram negative –Answer will appear pink/red...

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BIOS 280 FINAL Exam

• gram positive

–Answer will appear purple after a gram stain

• gram negative

–Answer will appear pink/red after a gram stain

• size

–Answer pinpoint, pinhead, small, medium, large

• shape

–Answer round or irregular

• color

–Answer beige, cream, yellow, red, pink, orange

• margins

–Answer smooth, indistinct, lobulated, lobate, irregular, filamentous

• consistency

–Answer dry (surface is dull), moist (surface is shiny), mucoid (surface is slimy)

• elevation

–Answer flat, dome-shaped, conical, cratered

• texture

–Answer fuzzy, rough-looking (not always applicable) 1 / 2

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• glucose mineral salt agar

–Answer simple, defined medium. To survive, organism needs to make all their cellular components from glucose and inorganic salts

• trypticase soy agar

–Answer undefined medium with soy products and proteins. Sup- ports growth for most bacteria, including those that require vitamins, pre-formed amino acids, or other growth factors

• blood agar

–Answer very rich medium, 5% sheep's blood is added to the agar before it is poured into the Petri dish.This agar allows evaluation of the type of hemolysis produced by microorganisms

• mannitol salts agar

–Answer selective for salt tolerant microorganisms and inhibits the growth of gram-negative bacteria. It is differential for organisms that ferment mannitol. The red agar will turn yellow if the organism can ferment mannitol

• eosin methylene blue

–Answer usually selective for gram-negative rods and usually inhibits the growth of gram-positive bacteria. It is differential for bacteria that can ferment lactose. The fermenters will produce dark purple colonies. If the fermenter produces a lot of acid as a waste product, the colony will have a green sheen. Non-fermenter colonies will be white or light pink

• macConkey

–Answer usually selective for gram-negative rods and inhibits the growth of gram-positive bacteria. it is differential for bacteria that can ferment lactose. A fermenter will produce pink/red colonies. A non-fermenter will be seen as beige or white.

• glucose phenol red broth

–Answer used to identify glucose fermenters. A tube contain- ing a fermenter will turn the pH indicator yellow. A non-fermenter will remain red

• agar deeps
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