C785 Biochemistry preassessment Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four complete PCR cycles?
(ANS - 16
What is the function of DNA polymerase in the process of PCR?(ANS - It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence.
What are possible blood types of the parents of a patient with blood type AB?(ANS - A and B
115 116 117 118 − Amino Acid Number Tyr Gly Thr Pro − Amino Acid Sequence TAC GGG ACA CCA − DNA Sequence
What is the amino acid in position 118 that results from a single nucleotide insertion immediately following the three guanines that encode for glycine 116?(ANS - Thr
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val).
What change in codons corresponds to this mutation?(ANS - GAA to GTA
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Which type of mammalian DNA damage repair requires the presence of the chromosome that is homologous to the damaged chromosome?(ANS - Recombination
Xeroderma pigmentosum (XP) is a recessive genetic disease that occurs when one or more of the genes that perform nucleotide excision repair are nonfunctional.
Why do XP patients have a much higher incidence of skin cancer than the general population?(ANS - The mutation rate of all other genes is higher due to failure to repair.
Which mRNA sequence results in a polypeptide that contains three amino acids?
(ANS - 5' - CUU GAU ACU UAG - 3'
Which template strand DNA sequence would result in the amino acid (Ser)?
(ANS - 5' - CGA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
(ANS - 5' - ATT CGA GAT GGC - 3'
Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated with chronic exposure to cocaine and contributes to drug addiction.
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