CHT EXAM AND STUDY GUIDE EXAM NEWEST 2025

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CHT EXAM AND STUDY GUIDE EXAM NEWEST 2025

TEST BANK| COMPLETE QUESTIONS AND CORRECT

DETAILED ANSWERS (VE RIFIED ANSWERS)

ALREADY GRADED A+| CERTIFIED HEMODIALYSIS

TECHNICIAN TEST BANK|| BRAND NEW!!

A PCR-RFLP assay is performed on DNA amplified from exon 2 of the HLA-DRB1 gene. The restriction enzyme chosen recognizes a single site contained in DRB1*0701 but in no other DRB1 allele. In a patient with the HLA type DRB1*0101/DRB1*0701 how many bands would be expected to appear in the RFLP gel analysis?

• One
• Two
• Three
• Four - ANSWER - c. Three

Linkage disequilibrium between HLA loci is correlated with...

• Physical distance between loci on chromosome 6
• Order of discovery of the loci
• ABO
• Sex - ANSWER - a. Physical distance between loci on chromosome 6

HLA typing has value in parentage testing because

• HLA loci are few in number
• HLA alleles are few in number 1 / 4

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• The polymorphism of HLA alleles is high
• HLA alleles do not exhibit classical Mendelian segregation -

ANSWER - c. The polymorphism of HLA alleles is high

Which change would increase Relative Risk for disease Y with HLA allele X?

• Fewer patients with the disease Y observed in the sampled population
• Fewer total individuals with HLA type X in the sampled population
• Fewer controls with HLA type X in the sampled population
• Fewer patients with disease Y but without HLA type X in the sampled

population - ANSWER - c. Fewer controls with HLA type X in the sampled population

Which of the following immunosuppressive drugs directly blocks IL-2 production?

• OKT3
• Cyclosporin
• Azathioprine
• Sirolimus - ANSWER - b. Cyclosporin

Which of the following immunosuppressive drugs directly eliminates T cells?

• OKT3
• Cyclosporin
• Azathioprine 2 / 4

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• Sirolimus - ANSWER - a. OKT3

Which of the following immunosuppressive drugs blocks the autocrine signal delivered through the IL-2/CD25 interaction?

• OKT3
• OKT3
• Azathioprine
• Sirolimus - ANSWER - d. Sirolimus

Channel shift in a flow cytometry crossmatch is determined by examining which histogram?

• FSC versus SSC
• FITC versus cell number
• CD20 versus CD3
• CD20 versus CD4 - ANSWER - b. FITC versus cell number

Patient serum X gave a 10-channel shift versus NHS in a flow cytometry crossmatch. How should this crossmatch be interpreted?

• Positive crossmatch
• Negative crossmatch
• Ambiguous crossmatch
• Depends upon the HLA lab and their SOP - ANSWER - d. Depends

upon the HLA lab and their SOP

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pg. 4 In a reverse SSO assay the genomic DNA is often incubated with sodium hydroxide before application to the membrane. Why?

• To clips the ends from the genomic DNA
• To remove homopolymer sequences
• To denature the DNA
• To remove non-standard bases - ANSWER - c. To denature the DNA

When pipetting the components of a PCR mix according to a recipe, which parameter is most important variable in achieving a successful PCR amplification?

• Pipetter Precision
• Pipetter Accuracy
• Pipetter Reproducibility
• Pipetter Resolution

e. Pipetter Display: Digital versus Analog - ANSWER - b. Pipetter

Accuracy

If 100g of NaCl (Na=22.99 g/Mole, Cl=35.43 g/Mole) are dissolved in 1500ml of water what is the molarity of the resulting solution?

• 0.23M
• 0.58M
• 0.75M
• 1.14M - ANSWER - d. 1.14M
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