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Genetic Engineering Questions and Answers | Latest Version | 2025/2026 | Correct & Verified
What is the primary tool used to cut DNA at specific sequences during genetic engineering?✔✔Restriction enzymes.
What is the role of ligase in genetic engineering?✔✔It joins fragments of DNA by forming phosphodiester bonds.
What is the name of the circular DNA used to transfer genes into bacteria?✔✔Plasmid.
What technique is used to amplify small segments of DNA?✔✔Polymerase Chain Reaction (PCR).
What is the purpose of gel electrophoresis in genetic engineering?✔✔To separate DNA fragments based on size.
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What is a genetically modified organism (GMO)?✔✔An organism that has been altered using genetic engineering techniques.
What is the term for inserting a gene from one species into another?✔✔Transgenesis.
Which enzyme is used to make complementary DNA (cDNA) from RNA?✔✔Reverse transcriptase.
What is CRISPR primarily used for in genetic engineering?✔✔Precise editing of DNA sequences.
What do scientists use as a guide in CRISPR-Cas9 systems?✔✔A short RNA sequence that matches the target DNA.
What is a gene knockout?✔✔A genetic technique in which a gene is completely disabled or removed.
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