Your Answer:
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BIOD 171 Microbiology Lab 3 Q & A
(Latest 2023 / 2024) Exam Portage Learning
List the four main steps used to prepare a WET mount, and indicate which (if any) step is optional.
Your Answer:
- Clean slide (70% ethanol)
- Circle area on slide with wax hydrophobic pen
3. Apply organism to slide:
• If from culture, use sterile loop to spread onto slide.• If from plate, use sterile loop to pick colonies and mix with a drop of distilled water.
- View under microscope.
- – Clean slide
- – Circle area on slide with wax/hydrophobic pen
- – Apply organism to slide
- – View under microscope
*Note: there are no optional steps in the wet mount.
Why is it important to first clean your slide before applying your sample?
Your Answer:
It is important to first clean your slide before applying the sample in order to remove contaminants from the slide.You must first remove any unwanted contaminants from the slide otherwise it may be difficult to distinguish between the pathogen of interest and a contaminant.
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