Question 1 Not yet graded / 3 pts Question 2 Not yet graded / 3 pts Question 3 Not yet graded / 4 pts BIOD 171 Microbiology Lab 8 Q & A
(Latest 2023 / 2024) Exam Portage Learning
The word ‘antigen’ is actually a combination for what two words?
Your Answer:
antibody generating Antibody-generating
For the ELISA assay depicted below, which step (A-E) first contains only specifically bound antigens (aka a 'pure' population)?
Your Answer:
C Specifically bound antigens are present in step C. Specific and non-specific antigens are present in B but then the wash step removes the non-specific (unbound) antigens, leaving only the specific population seen in step C.
What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step D (above)?
Your Answer:
It would be dark yellow. Color intensity depends on amount of antigens present. If the unbound secondary antigens are not washed away there will be a high amount of antigens present. 1 / 2
Question 4 Not yet graded / 2.5 pts Question 5 Not yet graded / 2.5 pts Question 6 Not yet graded / 2.5 pts Question 7 Not yet graded / 2.5 pts All of the wells would have a dark color. The intensity of the readout is dependent upon the amount of the labeled secondary antibody present in the well. Since the unbound secondary antibody was not washed away, all wells would react equally.
A western blot separates proteins based on .
Your Answer:
size Size
When running a western blot, what is the purpose of the marker?
Your Answer:
The marker is a standard and indicates a known protein size.The marker serves as molecular weight standards for the assay. The marker contains a collection of known molecular weight bands (also known as a molecular weight ladder) to be used in comparison to the samples being run on the gel.
When developing a western blot what two factors influence the intensity of the band?
Your Answer:
The amount of protein loaded and the time for development Both the amount of protein loaded as well as the duration (time) of development is directly proportional to band intensity.
Identify (specific name) given to the region of the western blot indicated by the arrow.
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