Labl Practicum:l BIOSl 242/l BIOS242l
(Latestl 2026/l 2027l Update)l Fundamentalsl ofl Microbiologyl withl Labl Guide|l Q/Al |l Gradel A|l 100%l Correctl (Verifiedl Answers)—Chamberlain
Q:l isolatingl purel cultures
Answer:
Disinfectl bench.Labell agarl plate.Sterilizel loop,l cool,l andl obtainl sample.Streakl samplel inl quadrantl 1.Flamel loop,l cool,l streakl quadrantl 2l (draggingl froml quadrantl 1).Repeatl forl quadrantsl 3l andl 4,l flamingl loopl betweenl each.Incubatel platel agarl sidel up.
Q:l stepsl forl graml staining
Answer:
1.l crystall violetl (primaryl stain) 2.l iodinel (mordant) 3.l alcoholl (decolorizer) 4.l safraninl (counterstain)
Q:l graml positivel bacteria 1 / 3
Answer:
retainl crystall violetl (purple)
Q:l graml negativel bacteria
Answer:
safraninl (pink/red)
Q:l cocci
Answer:
sphericall bacteria
Q:l bacilli
Answer:
Rod-shapedl bacteria
Q:l spirilla
Answer:
spiral-shapedl bacteria
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Q:l physicall methods
Answer:
heaat,l filtration,l radiation
Q:l chemicall methods
Answer:
disinfectants,l antiseptics
Q:l outcomes
Answer:
sterilization,l disinfection,l antispesis
Q:l phenoll redl broth
Answer:
carbohydratel fermentation
Q:l yellowl color
Answer:
acidl productionl (positivel fermentation)
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