pg. 1 Micro Lab Quiz 3/ Actual Exam Questions with Correct Verified Answers/ Graded A+
what tests would you perform for identifying a urine specimen - Correct Answer - 1.gram stain
- oxidase spot test
- indole spot test
what does the oxidase test test for? - Correct Answer - indophenol oxidase-an enzyme produced by an organism
signs of a possible kidney infection include: (4) - Correct Answer - -increased protein -blood present -nitrate positive -leukocyte esterase positive
urine culture for bacteria should be plated to... - Correct Answer - SBA/ BAP MAC ...in order to isolate pathogens
when is a stool specimen usually ordered? - Correct Answer - If a patient has diarrhea for long period of time or is extremely ill
How should a stool specimen be collected? - Correct Answer - In a clean container, does not have to be sterile (specimens are usually not formed (diarrhea, watery, bloody, mucous)
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pg. 2 How should a stool specimen be processed and transported? - Correct Answer - - processed within 2 hrs.-refrigerated if delayed -can use Cary Blair media for transport
What should be done if a parasite is suspected in a stool sample? - Correct Answer - order an ova and parasite exam or screen for common parasites like Giardia and Cryptosporidium
common causes of Salmonella, Shigella, Yersinia and Campylobacter - Correct Answer
- food/water poisoning...not normal flora of bowel
What biochemical tests should be performed on all isolates for stool samples - Correct Answer - 1. Triple Sugar Iron salt slant
- Urea broth or Urea slant
- MRVP broth
- Citrate slant
- subculture to SBA/BAP for gram stain, oxidase, and indole
TSI test - Correct Answer - Triple Sugar Iron Agar
What does the Indole spot test for? - Correct Answer - tryptophanase- an enzyme that produces indole in the presence of the amino acid tryptophan (in SBA)
reagents in Indole test - Correct Answer - p-dimethylaminocinnamaldehyde
Indole test procedure - Correct Answer - 1. drop reagent on filter paper
- smear organism on over reagent
- green on filter paper if positive
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