MLPAO Test Prep ~ Histology and Cytology Study Guide Histology Definition - Correct Answer Study of the structure of tissues Histology: Types of specimens received - Correct Answer Surgical procedures, biopsies, autopsies Histology: Surgical specimens include - Correct Answer Whole or part of an organ, cysts, tumors Histology: Biopsy specimens include - Correct Answer Removal of a representative sample (through needle aspiration, surgery, or endoscopy) Histology: Autopsy specimens include - Correct Answer Removal of tissue from a deceased person (to analyze the cause of death, diagnosis, or for research) Specimens must be labelled with - Correct Answer First and last name, DOB, pt address, Unique ID number, OHIP number, Specimen type, Specimen number Specimens are assigned a ____ number through LIS - Correct Answer Surgical Specimens must have a _______ with them - Correct Answer requisition Requisition / on-line request must contain the following - Correct Answer pt first and last name, DOB, Unique ID Number, OHIP number, pt Address, pt location, ordering physician, specimen type, specimen number (if there is more than one specimen) Specimens arrive in the lab as either a ___ or ____ specimen - Correct Answer Fixed, unfixed What is the first step when dealing with processing histology specimens? - Correct Answer Verify patient information of requisition Act in which components of cells and tissues are fixed in a physical and somewhat chemical state to endure treatment, avoiding loss, alteration, and decomposition - Correct Answer Fixation 1 / 2
Specimens should be placed in a fixative that is ____ the estimated volume of the tissue - Correct Answer 10-20X What is considered to be the universal fixative? - Correct Answer Formalin/formaldehyde Fixation preserves tissue in a - Correct Answer lifelike manner Fixation prevents - Correct Answer putrefaction and autolysis Autolysis definition - Correct Answer Decomposition due to the action of enzymes Putrefaction definition - Correct Answer Decomposition due to the action of bacteria Fixation denatures or precipitates ___ - Correct Answer Proteins
Fixation: Go through beneficial ___ - Correct Answer hardening
Fixation: Enhances ____ procedures - Correct Answer staining
Fixation: ____ microorganisms - Correct Answer fixes
Advantages of other fixatives: B5 - Correct Answer Displays good nuclear detail
Advantages of other fixatives: Glutaraldehyde - Correct Answer Used in electron microscopy Fixative that fixes tissues for cross-linking proteins - Correct Answer Glutaraldehyde Grossing of specimens Record the description of tissue including - Correct Answer Size, color, consistency, and any abnormalities (if present) Grossing of specimens tissues are - Correct Answer Sliced open and viewed
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