Thin Layer Chromatography –Questions/Answers ____ a method of separating mixtures into their pure components ✔️Ans - Chromatography chromatography: a method of _____ ✔️Ans - separating mixtures into their pure component Chromatography separates mixtures because the ____ between the various components of the mixture and the stationary phase are different ✔️Ans - intermolecular attractions Chromatography separates mixtures because the intermolecular interactions between the ____ ✔️Ans - various components of the mixture and the stationary phase are different Chromatography is a method of separation using a ___ and a ____ ✔️Ans - stationary phase; mobile phase separation occurs as the mobile phase ____ the stationary phase ✔️Ans - passes over the mobile phase can be ___ or ___ ✔️Ans - liquid; gas the stationary phase can be ___ or ___ ✔️Ans - solid; liquid
Chromatography-Basic Principle: same basic principle as TLC but now ____ of
pressure (_____) to ___ compounds ✔️Ans - use gravity; medium to high; elute Chromatography is both a ____ and ____ method ✔️Ans - qualitative; quantitative Chromatography is used for _____ the course of a reaction ✔️Ans - monitoring Chromatography is used for monitoring ____ ✔️Ans - the course of a reaction 1 / 2
- types of chromatography used for monitoring the course of a reaction
- / 2
✔️Ans - thin layer chromatography; gas chromatography; high performance liquid chromatography what does TLC stand for ✔️Ans - thin layer chromatography what does GC stand for ✔️Ans - gas chromatography what does HPLC stand for ✔️Ans - high performance liquid chromatography chromatography is used for _____ the components of a reaction ✔️Ans - isolating what methods of chromatography are used for isolating the components of a reaction ✔️Ans - column chromatography; prep HPLC; Prep TLC Chromatography is both a qualitative and quantitative method used for ___, ___ and ___ ✔️Ans - 1) monitoring the course of a reaction 2) isolating the components of a reaction 3) assisting in the identification of a compound In liquid chromotagraphy ____ is established ✔️Ans - equilibrium equation for chemical equilibrium is established and can be represented by the following equation ✔️Ans - in the equation for chemical equilibrium what does Xm and Xs stand for ✔️Ans - the concentrations of solute molecules in the mobile phase and the stationary phase in the equation for chemical equilibrium what does Sm and Ss stand for ✔️Ans - Sm and Ss are for the solvent molecule concentrations in the equation for chemical equilibrium what does the value K represent ✔️Ans - the distribution coefficient or equilibrium between the solute and solvent molecules and between the mobile and stationary phases