WGU 785 Final Exam (Latest 2023 - 2024)
New Full Exam Questions and Answers 100% Correct
- Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome
for their kids?
Answer: His daughters would be carriers. This is x-link recessive.
- Autosomal:Dominant:
Answer:
Autosomal: males and females equally affected.
Dominant: non-carrier parents
- polymerase chain reaction (PCR)
Answer: The process of copying DNA in the lab. Uses Template DNA, Nucleotides
(dNTPS), DNA Polymerase, and DNA primers.
- 3 Steps of PCR
- Annealing: reaction is cooled to 50C; primers stick to the DNA you want to copy
Answer : 1. Denaturation: DNA is heated to 95C to separate it.
and add DNA polymerase.
3. Elongation: reaction heated to 70C and DNA polymerase, adding nucleotides
building a new DNA strand.
- Base Excision Repair (BER)
- / 3
Answer: How you repair a mutation. BER is used to repair damage to a base caused by harmful molecules. You remove the base that is damaged and replace it. *BER removes a single nucleotide* DNA glycolsylase - sees damaged DNA and removes it.DNA polymerase-puts the right one back in while DNA ligase seals it.
6. Mismatch repair (MMR) occurs during:
Answer: replication. DNA polymerase proof- reads but sometimes a mismatch pair
gets through. MMR removes a large section of the nucleotides from the new DNA
and DNA polymerase tries again. (Ex: C-T instead of C-A)
- Mismatch Repair corrects what kind of DNA damage?
Answer: When a base is mis- matched due to errors in replication. Such as G-T instead of G-C. DNA polymerase comes by and fixes it.
- What happens when DNA polymerase binds to DNA to make RNA?
Answer: TRAN- SCRIPTION! DNA polymerase takes the individual nucleotides
and matches them to the parental sequences to ensure a correct pair. It must bind with RNA primer to work.
- What is needed for DNA replication?
Answer: DNA polymerase
- Nonsense Mutation
Answer: Change in 1 nucleotide produces a STOP codon Stop= nonsense because it
is no more.
- Silent Mutation
Answer: Change in 1 nucleotide but codes for the same amino acid. Silent= the
change doesn't change the name of the protein
- Missense Mutation
Answer: Change in 1 nucleotide leads to a code for a different amino acid. Missense 2 / 3
= mistake was made.
- What happends during RNA splicing?
Answer: During RNA splicing introns are cut out, the remaining exons are joined
together.
14. 5'ATG AGT CTC TCT 3'
Find the DNA template strand.
Answer: 3'TAC TCA GAG AGA 5'
The DNA template strand is complimentary. So start with the opposite number, then go L-R with the complimentary letter.
15. 5'ATG AGT CTC TCT 3'
What is the corresonding mRNA sequence?
Answer: 5'AUG AGU CUC UCU 3'
This sequence is the same as the coding strand except T changes to U because it is RNA. RNA doesn't have T.
- How would a mutation from CTC to ATC affect the protein sequence?
(CTC/ATC - coding strand, AUC - mRNA strand)
Answer: This will make a missense mutation because it changes the name of the
protein. (look at the chart provided.) missense = mistake
- DNA replication process
Answer: DNA ->Transcription -> RNA -> Translation -> Polypeptide
- Describe how you would find what ionized Alanine looks like.
- / 3
Answer: This is an amino acid. Look for the "R" group. Alanine is a hydrophobic amino acid that has CH3. It is a weak interaction. An ionized acid will have a + or - charge.