WGU C785 Biochemistry Exam 2 ( Latest 2023 – 2024 )
(ACTUAL EXAM ) Questions and Answers (Solved)
- polymerase chain reaction (PCR)
Answer: process to copy DNA
- What is needed to complete PCR
Answer: Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA
- Steps of PCR
Answer: denaturation, annealing, elongation
- Denaturation
Answer: loss of normal shape of a protein due to heat or other factor
- Annealing
Answer: cooled to 50c primers stick and want to copy and add DNA poly merase
- Elongation
Answer: reaction heated to 70C and DNA polymerase add nucleotides building a
new DNA strand.
- base excision repair
Answer: a modified base is first excised and then the entire nucleotide is replaced 1 / 2
- DNA glycosylase
Answer: removes damaged DNA
- DNA polymerase
Answer: replaces to damaged DNA ligase seals it
- base excision repair
Answer: removes one single nucleotide
- MIsmatch repair
Answer: The cellular process that uses special enzymes to fix incor rectly paired nucleotides.
- What DNA damage is corrected by mismatch repair
Answer: errors in replication fixed
- What occurs when DNA polymerase binds to DNA to make RNA
Answer: transcription ! DNA polymerase takes the individual nucleotides and
matches the them to the parental sequences to ensure a correct pair. It must bind with RNA primer to work.
- Nonsense
Answer: change in 1 nucleotide produces a STOP codon
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